The original article was provided by SSIbio (Scientific Specialties).
The IsoFreeze PCR rack plays a crucial role in maintaining the stability of samples during the qPCR process.
Sample Preservation:
Many protocols require the rapid processing of cells at near-physiological conditions to prevent unwanted alterations in gene expression or cell death. Cells are typically kept on ice or on an IsoFreeze PCR rack during collection and storage at -80 °C if not processed immediately. The IsoFreeze PCR rack helps maintain a stable and low-temperature environment, crucial for preserving the integrity of the samples.
Temperature Control:
During various steps of the qPCR protocol, including cell picking, lysis, and reverse transcription, maintaining consistent and appropriate temperatures is essential for the success of the experiment. The IsoFreeze PCR rack helps in achieving this by providing a uniform cooling surface, preventing fluctuations in sample temperature that could adversely affect the results.
Compatibility with High-Throughput Techniques:
The qPCR protocol aims for high-throughput single-cell transcriptome analysis. The IsoFreeze PCR rack is suitable for accommodating multiple tubes or 96-wells plates containing single cells, facilitating efficient processing of a large number of samples simultaneously.
Overall, the IsoFreeze PCR rack contributes to the success of qPCR by providing a stable, low-temperature environment necessary for preserving sample integrity and ensuring reliable results.
Scientific Specialties IsoFreezer PCR Rack is designed for laboratories, research facilities, and scientific settings, offering ideal storage for samples, reagents, and laboratory materials.
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